Фото: Григорий Сысоев / РИА Новости
Now that single-molecule detection was no longer necessary, Solexa was able to develop its signature sequencing chemistry. The process takes place on a chip called a flow cell, which contains a lawn of short DNA sequences affixed to its surface. The template DNA is broken up into small fragments, and adapter sequences, complementary to the DNA on the flow cell’s surface, are added to the ends of each fragment. DNA fragments are then passed over the flow cell, where the adapter sequences bind to spots on the DNA lawn. At this point, primers are added, and an initial round of amplification takes place: the short DNA sequences on the flow cell are extended to create sequences complementary to the bound template DNA fragments, which are then washed away. The sequences present in the fragments of template DNA are now affixed directly to the flow cell.
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起初，狗显然有些不适应，尤其是晚上回到房间以后，它便时不时要嚎叫一番。而它一有动静，隔壁的狗有时也要跟着吠，甚至于它嚎够歇菜后，“邻居”们又起了兴致，叫个没完。我一度担心，狗在这种环境里，能睡好么？当然，事实证明我想多了，夜深了，狗趴在沙发上睡着了，大概还在做梦奔跑，腿一抽一抽的。
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